cd40l blocking Search Results


human cd40l elisa kit  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH human cd40l elisa kit
Cathelicidins induce platelet activation and secretion. a – f Incubation of isolated human platelets for 15 min with increasing concentrations of LL-37 or scrambled control peptide (Scra; applied Scra concentration as indicated or equivalent to the maximal LL-37 concentration). ADP (5 µmol/L), collagen (Coll, 5 µg/mL), thrombin (Thr, Thrombin 0.05 U/mL), or GPVI receptor activating antibody HGP4C9 (1 µg/mL) were used for comparison. a Flow cytometric analysis of P-selectin surface expression ( n = 6). b <t>CD40L</t> release into the supernatant as measured by ELISA ( n = 3). Flow cytometry analysis of c <t>CD40</t> <t>ligand</t> (CD40L) surface expression ( n = 4), d intracellular IL-1β ( n = 5), e HMGB1 surface expression ( n = 4), and f GPIIb/IIIa receptor activation ( n = 6). g – i Flow cytometry analysis of CRAMP-induced activation of isolated mouse platelets. g P-selectin ( n = 5), h CD40L ( n = 4), and i activated GPIIb/IIIa ( n = 7). Thrombin (Thr, 0.5 U/mL) was used for comparison. Graphs show mean and SEM. P- values were determined by one-way repeated measures ANOVA with Bonferroni correction ( a , c – e ), paired t -test ( b , f ), or unpaired t -test ( g – i )
Human Cd40l Elisa Kit, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cd40l elisa kit/product/Biozol Diagnostica Vertrieb GmbH
Average 90 stars, based on 1 article reviews
human cd40l elisa kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
monoclonal blocking ab murine cd40l  (Macrozyme b.v)
90
Macrozyme b.v monoclonal blocking ab murine cd40l
Cathelicidins induce platelet activation and secretion. a – f Incubation of isolated human platelets for 15 min with increasing concentrations of LL-37 or scrambled control peptide (Scra; applied Scra concentration as indicated or equivalent to the maximal LL-37 concentration). ADP (5 µmol/L), collagen (Coll, 5 µg/mL), thrombin (Thr, Thrombin 0.05 U/mL), or GPVI receptor activating antibody HGP4C9 (1 µg/mL) were used for comparison. a Flow cytometric analysis of P-selectin surface expression ( n = 6). b <t>CD40L</t> release into the supernatant as measured by ELISA ( n = 3). Flow cytometry analysis of c <t>CD40</t> <t>ligand</t> (CD40L) surface expression ( n = 4), d intracellular IL-1β ( n = 5), e HMGB1 surface expression ( n = 4), and f GPIIb/IIIa receptor activation ( n = 6). g – i Flow cytometry analysis of CRAMP-induced activation of isolated mouse platelets. g P-selectin ( n = 5), h CD40L ( n = 4), and i activated GPIIb/IIIa ( n = 7). Thrombin (Thr, 0.5 U/mL) was used for comparison. Graphs show mean and SEM. P- values were determined by one-way repeated measures ANOVA with Bonferroni correction ( a , c – e ), paired t -test ( b , f ), or unpaired t -test ( g – i )
Monoclonal Blocking Ab Murine Cd40l, supplied by Macrozyme b.v, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal blocking ab murine cd40l/product/Macrozyme b.v
Average 90 stars, based on 1 article reviews
monoclonal blocking ab murine cd40l - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
blocking anti-cd40l 24-31; cd154; igg1  (Ansell Healthcare)
90
Ansell Healthcare blocking anti-cd40l 24-31; cd154; igg1
Inhibition of T lymphocyte-mediated activation of DC by mAb. DC were incubated for 16 hr with (a) autologous T lymphocytes with 10 ng/ml SEA or (b) allogeneic T lymphocytes in the presence of isotype-matched mAb controls or mAb to DC or T lymphocyte surface antigens. DC were identified in these cocultures with labelling with HLA-DR-PE-Cy5. The levels of CD86 (a,b) and CD80 (b) expression were determined on the DR++/CMFDA− DC by labelling in a third colour. Plots show the means of four individual experiments (±SEM) with the CD80 and CD86 MFI values of cocultured DC normalized for each experiment to the control cocultures without mAb (100%; dotted line). Statistically significant differences (by the two-tailed unpaired Student’s t-test) are indicated for: <t>**IgG1</t> versus <t>CD40L,</t> P<0·01 and *IgG2a versus HLA-DR, P<0·02 and (b); IgG2a versus HLA-DR; **P<0·01, *P<0·02 for CD80 and CD86 levels, respectively).
Blocking Anti Cd40l 24 31; Cd154; Igg1, supplied by Ansell Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blocking anti-cd40l 24-31; cd154; igg1/product/Ansell Healthcare
Average 90 stars, based on 1 article reviews
blocking anti-cd40l 24-31; cd154; igg1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Cathelicidins induce platelet activation and secretion. a – f Incubation of isolated human platelets for 15 min with increasing concentrations of LL-37 or scrambled control peptide (Scra; applied Scra concentration as indicated or equivalent to the maximal LL-37 concentration). ADP (5 µmol/L), collagen (Coll, 5 µg/mL), thrombin (Thr, Thrombin 0.05 U/mL), or GPVI receptor activating antibody HGP4C9 (1 µg/mL) were used for comparison. a Flow cytometric analysis of P-selectin surface expression ( n = 6). b CD40L release into the supernatant as measured by ELISA ( n = 3). Flow cytometry analysis of c CD40 ligand (CD40L) surface expression ( n = 4), d intracellular IL-1β ( n = 5), e HMGB1 surface expression ( n = 4), and f GPIIb/IIIa receptor activation ( n = 6). g – i Flow cytometry analysis of CRAMP-induced activation of isolated mouse platelets. g P-selectin ( n = 5), h CD40L ( n = 4), and i activated GPIIb/IIIa ( n = 7). Thrombin (Thr, 0.5 U/mL) was used for comparison. Graphs show mean and SEM. P- values were determined by one-way repeated measures ANOVA with Bonferroni correction ( a , c – e ), paired t -test ( b , f ), or unpaired t -test ( g – i )

Journal: Nature Communications

Article Title: Cathelicidins prime platelets to mediate arterial thrombosis and tissue inflammation

doi: 10.1038/s41467-018-03925-2

Figure Lengend Snippet: Cathelicidins induce platelet activation and secretion. a – f Incubation of isolated human platelets for 15 min with increasing concentrations of LL-37 or scrambled control peptide (Scra; applied Scra concentration as indicated or equivalent to the maximal LL-37 concentration). ADP (5 µmol/L), collagen (Coll, 5 µg/mL), thrombin (Thr, Thrombin 0.05 U/mL), or GPVI receptor activating antibody HGP4C9 (1 µg/mL) were used for comparison. a Flow cytometric analysis of P-selectin surface expression ( n = 6). b CD40L release into the supernatant as measured by ELISA ( n = 3). Flow cytometry analysis of c CD40 ligand (CD40L) surface expression ( n = 4), d intracellular IL-1β ( n = 5), e HMGB1 surface expression ( n = 4), and f GPIIb/IIIa receptor activation ( n = 6). g – i Flow cytometry analysis of CRAMP-induced activation of isolated mouse platelets. g P-selectin ( n = 5), h CD40L ( n = 4), and i activated GPIIb/IIIa ( n = 7). Thrombin (Thr, 0.5 U/mL) was used for comparison. Graphs show mean and SEM. P- values were determined by one-way repeated measures ANOVA with Bonferroni correction ( a , c – e ), paired t -test ( b , f ), or unpaired t -test ( g – i )

Article Snippet: Platelet CD40L secretion was measured using a human CD40L ELISA kit (Biozol Diagnostica, Houston, USA).

Techniques: Activation Assay, Incubation, Isolation, Control, Concentration Assay, Comparison, Expressing, Enzyme-linked Immunosorbent Assay, Flow Cytometry

Inhibition of T lymphocyte-mediated activation of DC by mAb. DC were incubated for 16 hr with (a) autologous T lymphocytes with 10 ng/ml SEA or (b) allogeneic T lymphocytes in the presence of isotype-matched mAb controls or mAb to DC or T lymphocyte surface antigens. DC were identified in these cocultures with labelling with HLA-DR-PE-Cy5. The levels of CD86 (a,b) and CD80 (b) expression were determined on the DR++/CMFDA− DC by labelling in a third colour. Plots show the means of four individual experiments (±SEM) with the CD80 and CD86 MFI values of cocultured DC normalized for each experiment to the control cocultures without mAb (100%; dotted line). Statistically significant differences (by the two-tailed unpaired Student’s t-test) are indicated for: **IgG1 versus CD40L, P<0·01 and *IgG2a versus HLA-DR, P<0·02 and (b); IgG2a versus HLA-DR; **P<0·01, *P<0·02 for CD80 and CD86 levels, respectively).

Journal:

Article Title: Induction of dendritic cell costimulator molecule expression is suppressed by T cells in the absence of antigen-specific signalling: role of cluster formation, CD40 and HLA-class II for dendritic cell activation

doi: 10.1046/j.1365-2567.1999.00860.x

Figure Lengend Snippet: Inhibition of T lymphocyte-mediated activation of DC by mAb. DC were incubated for 16 hr with (a) autologous T lymphocytes with 10 ng/ml SEA or (b) allogeneic T lymphocytes in the presence of isotype-matched mAb controls or mAb to DC or T lymphocyte surface antigens. DC were identified in these cocultures with labelling with HLA-DR-PE-Cy5. The levels of CD86 (a,b) and CD80 (b) expression were determined on the DR++/CMFDA− DC by labelling in a third colour. Plots show the means of four individual experiments (±SEM) with the CD80 and CD86 MFI values of cocultured DC normalized for each experiment to the control cocultures without mAb (100%; dotted line). Statistically significant differences (by the two-tailed unpaired Student’s t-test) are indicated for: **IgG1 versus CD40L, P<0·01 and *IgG2a versus HLA-DR, P<0·02 and (b); IgG2a versus HLA-DR; **P<0·01, *P<0·02 for CD80 and CD86 levels, respectively).

Article Snippet: Blocking anti-CD40L (clone 24-31; CD154; IgG1) was from Ansell Corporation (Bayport, MN).

Techniques: Inhibition, Activation Assay, Incubation, Expressing, Two Tailed Test

Effect of class II and CD40 cross-linking on DC costimulator expression. (a) DC were incubated with SEA (1 μg/ml), CD40 ligand trimer (CD40LT; 10 μg/ml) or in medium alone (MED) for the indicated times, then labelled with CD86 mAb plus FITC-SAM. Data are from one of two similar time–courses. (b) the biological activity of SEA was checked by incubating 5×104 PBMC per well with the indicated concentrations of SEA or phytohaemagglutinin (5 μg/ml) for 3 days prior to pulsing with 0·5 μCi/well of thymidine for 16 hr. Results are plotted as means of triplicate wells±SD. Representative of two experiments performed.

Journal:

Article Title: Induction of dendritic cell costimulator molecule expression is suppressed by T cells in the absence of antigen-specific signalling: role of cluster formation, CD40 and HLA-class II for dendritic cell activation

doi: 10.1046/j.1365-2567.1999.00860.x

Figure Lengend Snippet: Effect of class II and CD40 cross-linking on DC costimulator expression. (a) DC were incubated with SEA (1 μg/ml), CD40 ligand trimer (CD40LT; 10 μg/ml) or in medium alone (MED) for the indicated times, then labelled with CD86 mAb plus FITC-SAM. Data are from one of two similar time–courses. (b) the biological activity of SEA was checked by incubating 5×104 PBMC per well with the indicated concentrations of SEA or phytohaemagglutinin (5 μg/ml) for 3 days prior to pulsing with 0·5 μCi/well of thymidine for 16 hr. Results are plotted as means of triplicate wells±SD. Representative of two experiments performed.

Article Snippet: Blocking anti-CD40L (clone 24-31; CD154; IgG1) was from Ansell Corporation (Bayport, MN).

Techniques: Expressing, Incubation, Activity Assay